Cloning of Purine Nucieoside TH 6 - 2 and CharacterizationPhosphorylase ll Gene of Its Gene
نویسندگان
چکیده
enzyme (Pu-NPase ID is a mi-or enzyme that eMciently phosphorolyzes adienosine and its deriyatiyes ratheT tha" Gther puri"e rmcleosides ]ike Escherichia coti Pu-NPase. The gene coding for Pu-NPase II (punB' gene) has beelt clloned andi sequenced from TH 6-2 strain. The deduced amine acid sequence of Pu-NPase II shared 54`M, iderwity with that of E. coii enzyme, while it had no significant homelogy to that of Pu-NPasE・ I er euknryotic enzymes. By producing the Pu-NPase II in E. coti cells, the Pu-NPase II has been purified and eharaeterkzed.
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